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Updated: Oct 11, 2025

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Genetic diversity of Norway spruce ecotypes assessed by GBS-derived SNPs
Jiří Korecký1, Jaroslav Čepl2, Jan Stejskal2
1Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences, Kamýcká 129, Suchdol, 165 00, Praha, Czech Republic. korecky@fld.czu.cz.
Abstract:
We investigated the genetic structure of three phenotypically distinct ecotypic groups of Norway spruce (Picea abies) belonging to three elevational classes; namely, low- (acuminata), medium- (europaea), and high-elevation (obovata) form, each represented by 150 trees. After rigorous filtering, we used 1916 Genotyping-by-Sequencing generated SNPs for analysis. Outputs from three multivariate analysis methods (Bayesian clustering algorithm implemented in STRUCTURE, Principal Component Analysis, and the Discriminant Analysis of Principal Components) indicated the presence of a distinct genetic cluster representing the high-elevation ecotypic group. Our findings bring a vital message to forestry practice affirming that artificial transfer of forest reproductive material, especially for stands under harsh climate conditions, should be considered with caution.
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