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Fruit Derived Potentially Bioactive Bioengineered Silver Nanoparticles.

Abu Baker1, Sana Iram2, Asad Syed3

  • 1Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026, India.

International Journal of Nanomedicine
|December 1, 2021
PubMed
Summary

This study synthesized silver nanoparticles (AgNPs) using Benincasa hispida fruit proteins. The biogenic AgNPs demonstrated significant antibacterial, antibiofilm, and anticancer properties against lung cancer cells.

Keywords:
AgNPsBenincasa hispidaantibacterialantibiofilmanticancergreen synthesis

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Materials Science

Background:

  • Protein-directed synthesis of inorganic nanoparticles offers biocompatibility and stability.
  • Biogenic nanoparticles are gaining attention for diverse applications.
  • Proteins provide a reducing environment and encapsulation for nanoparticle synthesis.

Purpose of the Study:

  • To synthesize silver nanoparticles (AgNPs) using Benincasa hispida fruit proteins.
  • To evaluate the antibacterial, antibiofilm, and anticancer activities of the synthesized AgNPs.
  • To assess the biocompatibility and mechanism of action of the AgNPs.

Main Methods:

  • Synthesis of AgNPs using Benincasa hispida fruit proteins at 37°C for five days.
  • Characterization of AgNPs using UV-Vis spectroscopy, TEM, zeta potential, DLS, and FTIR.
  • Evaluation of antibacterial activity (MIC), antibiofilm activity, and anticancer activity (MTT assay) against A549 and NRK cell lines.

Main Results:

  • TEM analysis confirmed AgNPs with an average size of 27 ±1 nm, with protein encapsulation verified by FTIR.
  • AgNPs exhibited potent antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteric, and Staphylococcus epidermis.
  • AgNPs effectively inhibited biofilm formation and demonstrated significant anticancer activity against A549 lung cancer cells with minimal toxicity to normal NRK cells.

Conclusions:

  • Successfully synthesized potent biogenic silver nanoparticles (AgNPs) using plant proteins.
  • The synthesized AgNPs possess significant antibacterial, antibiofilm, and anticancer properties.
  • These findings highlight the potential of biogenic AgNPs in therapeutic applications.