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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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Published on: March 9, 2022

Modulating the chemo-mechanical response of structured DNA assemblies through binding molecules.

Chanseok Lee¹, Young-Joo Kim¹, Kyung Soo Kim²

¹Institute of Advanced Machines and Design, Seoul National University, Seoul 08826, Korea.

Nucleic Acids Research

|December 1, 2021

Summary

This summary is machine-generated.

Controlling DNA nanostructure shape and flexibility is key for molecular machines. This study shows DNA-binding molecules can tune these properties, enabling dynamic control of nanostructures.

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Designing a Bio-responsive Robot from DNA Origami

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Published on: July 8, 2013

Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

DNA nanotechnology enables complex DNA assemblies, but controlling their global shape and mechanical properties remains a challenge.
DNA-binding molecules can alter DNA's geometrical and mechanical characteristics at the strand level.

Purpose of the Study:

To demonstrate that DNA-binding ligands can systematically modulate the global shape and stiffness of DNA origami nanostructures.
To explore the chemo-mechanical response of DNA nanostructures for on-demand functionalization.

Main Methods:

Application of various DNA-binding drugs and fluorophores to straight and curved DNA origami bundles.
Analysis of changes in bending persistence length and radius of curvature of the nanostructures.

Main Results:

Demonstrated fast, recoverable, and controllable alterations in the global shape and stiffness of DNA nanostructures.
Showcased the effectiveness of selected ligands and concentrations in modulating nanostructure properties.

Conclusions:

Chemo-mechanical modulation using DNA-binding molecules offers a powerful method for reconfigurable and dynamic actuation of DNA nanomachineries.
This approach provides a versatile tool for designing advanced DNA-based molecular machines with tunable functionalities.