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The carbohydrate-active enzyme database: functions and literature.

Elodie Drula1,2, Marie-Line Garron1,2, Suzan Dogan1,2

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The Carbohydrate-Active enZymes (CAZy) database classifies enzymes, aiding research with regular updates and new family classifications. It provides essential functional information and taxonomic navigation for researchers in enzymology and biotechnology.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Bioinformatics

Background:

  • The Carbohydrate-Active enZymes (CAZy) database has been a cornerstone for enzyme classification for over 20 years.
  • It relies on human curation for classifying carbohydrate-active enzymes based on sequence families.
  • The database is crucial in the current era of large-scale sequencing and high-throughput biology.

Purpose of the Study:

  • To review the CAZy database's role and evolution over the last 8 years.
  • To highlight recent updates, including novel families and enhanced annotations.
  • To emphasize the integration of biochemical data from scientific literature.

Main Methods:

  • Continuous human curation of enzyme sequences.
  • Regular updates to the CAZy database classification system.
  • Integration and presentation of biochemical data from published literature.

Main Results:

  • Significant increase in novel enzyme families and annotations over the past 8 years.
  • Implementation of changes for improved taxonomic navigation.
  • Availability of comprehensive annotation downloads and literature-based biochemical data.

Conclusions:

  • The CAZy database remains a vital, curated resource for carbohydrate-active enzyme research.
  • Ongoing curation and integration of biochemical data enhance its utility for the scientific community.
  • The database facilitates research in enzymology, biotechnology, and related fields through accessible, up-to-date information.