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Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding.

Francesco Brunori^1,2, Deepak Kumar Padhi¹, Israel Alshanski¹

¹Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904, Israel.

Chembiochem : a European Journal of Chemical Biology

|December 1, 2021

Summary

This study shows how the sulfation degree of hyaluronan and iron ions (Fe3+) together influence interleukin-8 binding. These factors synergistically tune the electrochemical properties of surfaces interacting with this immune-activating cytokine.

Keywords:

electrochemistry interactions interleukin-8 metal binding sulfation pattern

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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

Area of Science:

Biochemistry
Materials Science
Immunology

Background:

Cytokines like interleukin-8 (IL-8) are crucial for immune responses during infection.
IL-8 interacts with sulfated glycosaminoglycans, with specific sulfation patterns influencing these interactions.
Metal ion binding can mediate and tune glycan-protein interactions on surfaces.

Purpose of the Study:

To investigate the combined effect of hyaluronan sulfation degree and Fe3+ on IL-8 binding.
To understand how these factors modulate surface-based glycan-protein interactions.

Main Methods:

Electrochemical impedance spectroscopy was employed to measure binding.
Surface characterizations were performed to analyze the interactions.
Hyaluronan with varying sulfation degrees and Fe3+ were used as experimental variables.

Main Results:

Both hyaluronan sulfation degree and Fe3+ significantly influenced IL-8 binding.
A synergistic effect was observed between sulfation degree and Fe3+ in modulating binding.
These combined factors tuned the electrochemical response of the glycated surfaces.

Conclusions:

The sulfation pattern of glycosaminoglycans and metal ion presence are critical for controlling cytokine interactions.
This synergistic interaction offers a method for tuning glycan-protein interactions on surfaces for potential applications in immunology and biomaterials.