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Protein Diffusion in the Membrane

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

Membrane Fluidity

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Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.

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Related Experiment Video

Updated: Oct 11, 2025

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

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Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins.

Amanda Buyan¹, Ben Corry²

¹Research School of Biology, Australian National University, Canberra, ACT, Australia. amanda.buyan@anu.edu.au.

Methods in Molecular Biology (Clifton, N.J.)

|December 2, 2021

Summary

Coarse-grained simulations offer a powerful method for studying membrane proteins. This guide details setting up these simulations to mimic cellular membrane environments effectively.

Keywords:

Lipids Membrane protein Membranes Molecular dynamics simulation

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Area of Science:

Computational Biology
Biophysics
Molecular Modeling

Background:

Molecular dynamics (MD) simulations are crucial for investigating molecular phenomena.
Atomistic simulations are limited by system size and timescale.
Coarse-grained (CG) simulations simplify systems for broader exploration.

Purpose of the Study:

To provide a comprehensive guide for setting up CG simulations.
To enable the study of membrane-bound proteins in realistic lipid bilayers.
To facilitate research in areas challenging for experimental methods.

Main Methods:

Utilizing coarse-grained modeling techniques.
Simulating proteins within a lipid bilayer environment.
Describing necessary procedures and equipment for setup.

Main Results:

Demonstration of a methodology for CG simulations of membrane proteins.
Creation of a simulation setup mimicking diverse membrane environments.
Enabling longer timescales and larger system sizes compared to atomistic methods.

Conclusions:

Coarse-grained simulations are effective for studying membrane protein behavior.
The described methods allow for the investigation of complex molecular systems.
This approach enhances the capabilities of computational studies in biophysics.