Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmon Mode-Selective Gold Nanodimers with a Metal-Semiconductor Hybrid Junction.

ACS nano·2026
Same author

Phosphatase and tensin homolog mRNA complexed with hyaluronated lipid nanoparticles for transdermal cancer immunotherapy.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Poly(dl-Alanine-<i>co</i>-Glycine): Peptide Analogue of Poly(dl-Lactide-<i>co</i>-Glycolide).

Biomacromolecules·2025
Same author

33 Unresolved Questions in Nanoscience and Nanotechnology.

ACS nano·2025
Same author

Dynamic birefringence and chirality of magnetically controllable assemblies of anisotropic plasmonic nanoparticles in dispersion.

Nature communications·2025
Same author

Iron Oxide Nanoparticles Modified with Galloylated DNA for Magnetically Enhanced DNA-Directed Assembly.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Related Experiment Video

Updated: Oct 11, 2025

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

3.5K

Hypothermic Stem Cell Storage Using a Polypeptide Thermogel.

Zhengyu Piao1, Jin Kyung Park1, So-Jung Park1

  • 1Department of Chemistry and Nanoscience, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea.

Biomacromolecules
|December 2, 2021
PubMed
Summary

A novel polypeptide thermogel enables stem cell storage at room temperature. This thermogel significantly improves stem cell recovery and health compared to conventional methods, offering a promising solution for cell transport and banking.

More Related Videos

Freezing and Thawing Human Embryonic Stem Cells
08:49

Freezing and Thawing Human Embryonic Stem Cells

Published on: December 24, 2009

46.1K
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

12.9K

Related Experiment Videos

Last Updated: Oct 11, 2025

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

3.5K
Freezing and Thawing Human Embryonic Stem Cells
08:49

Freezing and Thawing Human Embryonic Stem Cells

Published on: December 24, 2009

46.1K
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

12.9K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Hypothermic storage is crucial for stem cell preservation.
  • Current methods face challenges in maintaining cell viability and function.
  • Ambient temperature storage offers logistical advantages.

Purpose of the Study:

  • To develop a novel polypeptide-based thermogel for ambient temperature stem cell storage.
  • To evaluate the efficacy of the thermogel in preserving stem cell viability and function.
  • To compare the thermogel's performance against commercial preservation solutions.

Main Methods:

  • Stem cells were suspended in a poly(ethylene glycol)-poly(l-alanine) (PEG-PA) thermogel solution.
  • The sol-to-gel transition was induced by heating to 25 °C for storage.
  • Cell recovery was achieved via cooling-induced gel-to-sol transition.
  • Stem cell viability, biomarker expression, proliferation, and differentiation were assessed.

Main Results:

  • The PEG-PA thermogel demonstrated significantly higher stem cell recovery rates (64%) compared to HypoThermosol FRS (26%) after 7 days at 25 °C.
  • Stem cells stored in PEG-PA thermogel maintained superior expression of stemness biomarkers (NANOG, OCT4, SOX2), proliferation rates, and differentiation potentials.
  • Control thermogels (PBS and Pluronic F127) resulted in over 99% stem cell death under the same conditions.
  • Membrane stabilization was identified as a potential cell protection mechanism.

Conclusions:

  • The PEG-PA thermogel is an effective and cytocompatible tool for ambient temperature stem cell storage.
  • This thermogel system offers a convenient method for cell recovery, surpassing commercial preservation solutions.
  • The findings suggest the PEG-PA thermogel is a promising candidate for cell transportation and short-term banking applications.