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Lymphocyte migration in the adoptive transfer of EAU
Investigative Ophthalmology & Visual Science
|April 1, 1986
Summary
Transferring experimental autoimmune uveoretinitis (EAU) involves a small number of indium-111 labeled lymphocytes reaching the eye. Most cells migrate to other organs, but eyes with EAU show significantly more labeled cells.
Area of Science:
- Immunology
- Ophthalmology
- Cellular Biology
Background:
- Experimental autoimmune uveoretinitis (EAU) is an autoimmune disease affecting the eye.
- Understanding lymphocyte migration is crucial for autoimmune disease research.
Purpose of the Study:
- To track the migration of lymphocytes involved in the adoptive transfer of EAU.
- To quantify the number of lymphocytes reaching the eye during EAU induction.
Main Methods:
- Indium-111 labeled lymphocytes from S-antigen-immunized Lewis rats were adoptively transferred into naive rats.
- Lymphocyte migration was monitored using gamma camera imaging and radioactivity measurements in organs.
- EAU development was assessed in recipient rats.
Main Results:
- The majority of transferred lymphocytes left the peritoneal cavity within 24 hours, migrating to the liver, spleen, and thymus.
- Only a small fraction of labeled lymphocytes reached the eye.
- Eyes that developed EAU had significantly more labeled lymphocytes compared to control eyes.
Conclusions:
- Adoptive transfer of EAU is a complex process.
- A limited number of transferred lymphocytes are responsible for inducing uveoretinitis in the eye.