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Related Experiment Videos

Gene duplication in Saccharomyces cerevisiae.

P E Hansche, V Beres, P Lange

    Genetics
    |April 1, 1978
    PubMed
    Summary

    Researchers identified five duplications of the acid-phosphatase structural gene (acp1) in yeast. These duplications, often involving gene transposition, showed varied stability, with some exhibiting high fidelity and others instability during cell division.

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    Area of Science:

    • * Molecular Biology
    • * Yeast Genetics
    • * Genomics

    Background:

    • * Investigating gene duplication mechanisms is crucial for understanding genome evolution and adaptation.
    • * The acid-phosphatase structural gene (acp1) in S. cerevisiae is a model for studying gene amplification under selective pressure.

    Purpose of the Study:

    • * To characterize spontaneous and induced duplications of the acp1 gene in yeast.
    • * To analyze the stability and transmission fidelity of these gene duplications.

    Main Methods:

    • * Utilized chemostat cultures of S. cerevisiae under selection for hyper-phosphatase activity.
    • * Employed UV radiation to induce gene duplications.
    • * Analyzed gene duplication events, including transposition and chromosomal location, using genetic techniques.

    Main Results:

    • * Five independent duplications of the acp1 gene were recovered, with three induced by UV and two spontaneous.
    • * All duplications involved transposition of acp1 and adjacent genes to chromosome termini.
    • * One duplication exhibited high mitotic and meiotic transmission fidelity, while four were unstable (2% loss per mitosis).
    • * Selection successfully increased mitotic transmission fidelity in an unstable strain.
    • * No tandem duplications of acp1 were observed.

    Conclusions:

    • * Gene duplication, particularly involving transposition, is a significant mechanism for rapid adaptation in yeast under selective pressure.
    • * The stability of duplicated gene segments can vary considerably, influencing their long-term persistence.
    • * Directed selection can enhance the mitotic stability of unstable gene duplications.

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