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Published on: July 6, 2022
Dual-functional antibacterial and osteogenic nisin-based layer-by-layer coatings
Charlotte Roupie1, Béatrice Labat2, Sandrine Morin-Grognet2
1Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, Bd Maurice de Broglie, 76821 Mont Saint Aignan Cedex, France; Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 55 rue Saint-Germain, 27000 Évreux, France.
Dual-functional coatings combining chondroitin sulfate A (CSA) and poly-l-lysine (PLL) with nisin Z offer antibacterial and osteogenic properties. These advanced biomaterials promote bone healing while preventing bacterial infections in bone surgery.
Area of Science:
- Biomaterials Science
- Orthopedic Surgery
- Nanotechnology
Background:
- Implanted biomaterials are crucial in bone surgery, requiring surfaces that resist bacteria and promote bone cell growth for osseointegration.
- Current challenges involve developing materials with both antibacterial and osteogenic capabilities simultaneously.
Purpose of the Study:
- To design and fabricate dual-functional Layer-by-Layer (LbL) coatings with antibacterial and osteogenic properties for bone applications.
- To evaluate the efficacy of these coatings in preventing bacterial growth and supporting bone cell functions.
Main Methods:
- Fabrication of ultrathin LbL coatings using biocompatible polyelectrolytes: chondroitin sulfate A (CSA) and poly-l-lysine (PLL).
- Crosslinking of coatings with genipin (GnP) to enhance stability and mechanical properties.
- Incorporation of nisin Z, an antimicrobial peptide (AMP), for antibacterial activity against Gram-positive bacteria.
Main Results:
- The developed GnP-crosslinked CSA/PLL coatings demonstrated significant bactericidal effects against Staphylococcus aureus.
- Bone cell adhesion, proliferation, and osteogenic differentiation were fully maintained on the functionalized surfaces.
- The coatings exhibited enhanced resistance to environmental changes and suitable mechanical properties for bone cell interactions.
Conclusions:
- Ultrathin, dual-functional LbL coatings offer a promising strategy for developing advanced biomaterials in bone surgery.
- These coatings effectively combine antibacterial and osteogenic functionalities, addressing key challenges in osseointegration and infection prevention.
