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A ratiometric fluorescence sensor for 5-hydroxymethylfurfural detection based on strand displacement reaction
Nan Jiang1, Peng Li2, Shihao Sun2
1Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.
Talanta
|December 3, 2021
Summary
A new ratiometric fluorescence sensor enables highly sensitive detection of 5-hydroxymethylfurfural (HMF), a harmful food contaminant. This method uses strand displacement and magnetic separation for accurate HMF analysis in food samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- 5-hydroxymethylfurfural (HMF) is a food contaminant with potential health risks.
- Highly sensitive and selective detection methods for HMF are crucial for food safety.
Purpose of the Study:
- To develop a novel ratiometric fluorescence sensor for sensitive and selective HMF detection.
- To utilize strand displacement reaction (SDR) and magnetic separation for HMF analysis.
Main Methods:
- A ratiometric fluorescence sensor was designed using MnO2 nanosheets, aldehyde-functionalized DNA (S0-CHO), and amino-functionalized magnetic beads (NH2-MBs).
- HMF competed with S0-CHO for binding to NH2-MBs, followed by magnetic separation.
- Strand displacement reaction was employed to trigger fluorescence signals (FAM and Cy5) based on HMF presence.
Main Results:
- The sensor demonstrated high sensitivity with a linear detection range from 5 nM to 5 μM.
- The method exhibited excellent selectivity against potential interfering substances.
- The sensor was successfully applied to detect HMF in real food samples.
Conclusions:
- A novel, sensitive, and selective ratiometric fluorescence sensor for HMF detection was successfully developed.
- The method shows promise for application in food safety, environmental monitoring, and detection of other aldehyde-containing molecules.
