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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Published on: March 3, 2017
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Serum Exosomal microRNA Profiling in AIDS Complicated with Talaromyces marneffei Infection.
Qiu-Yue Ning1, Na Liu2, Ji-Zhou Wu1
1Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
Infection and Drug Resistance
|December 3, 2021
Summary
Three serum exosomal microRNAs (miRNAs) show potential as early diagnostic biomarkers for AIDS combined with Tuberculosis (TB) infection. These miRNAs, including miR-192-5p, miR-194-5p, and miR-1246, may aid in identifying coinfection.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Acquired Immunodeficiency Syndrome (AIDS) coinfection with Tuberculosis (TB) requires early diagnostic markers.
- Serum exosomal microRNAs (miRNAs) are investigated as potential biomarkers.
Purpose of the Study:
- To identify and analyze serum exosomal miRNAs for early diagnosis of AIDS combined with TB infection.
- To explore the diagnostic value of specific miRNAs in this coinfection.
Main Methods:
- RNA sequencing of serum exosomes from AIDS patients with and without TB infection.
- Validation using quantitative real-time polymerase chain reaction (qRT-PCR).
- Bioinformatic analysis to predict target genes and pathways.
Main Results:
- 131 differentially expressed serum exosomal miRNAs identified.
- miR-192-5p, miR-194-5p, and miR-1246 were upregulated in AIDS with TB patients.
- Combined ROC analysis showed an AUC of 0.742 for these three miRNAs.
Conclusions:
- miR-192-5p, miR-194-5p, and miR-1246 are potential biomarkers for AIDS combined with TB infection.
- These miRNAs are involved in key signaling pathways like TGF-β, AMPK, Wnt, MAPK, and cGMP-PKG/cAMP.
- Further research is needed to elucidate their biological functions and mechanisms.
