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Updated: Oct 11, 2025
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Published on: March 3, 2017
Serum Exosomal microRNA Profiling in AIDS Complicated with Talaromyces marneffei Infection
Qiu-Yue Ning1, Na Liu2, Ji-Zhou Wu1
1Department of Infectious Diseases, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
Introduction:
In order to find the early diagnostic markers of AIDS combined with TM infection, we detected and analyzed the serum exosomal miRNAs of AIDS patients with or without TM infection.
Materials And Methods:
We profiled the expression levels of miRNAs via RNA sequencing in serum exosomes from the pooled samples of 17 AIDS patients combined without TM infection and 15 AIDS combined with TM infection patients. For external validation, we validated these results using real-time quantification polymerase chain reaction (qRT-PCR) in an independent cohort of 35 AIDS patients combined without TM infection and 33 AIDS combined with TM infection patients. Finally, bioinformatics was used to predict the target genes and pathways of meaningful miRNAs.
Results:
A total of 131 serum exosomal miRNAs including 73 up-regulated and 59 down-regulated miRNAs were found to be differentially expressed (log2FC≥1 and FDR <0.01) in the two groups. A validation analysis revealed that three miRNAs (miR-192-5p, miR-194-5p and miR-1246) were upregulated in exosomes from AIDS combined with TM infection patients. ROC analyses showed that the AUC in combined diagnosis of the three miRNAs was 0.742, and the diagnostic sensitivity and specificity were 0.568 and 0.861, respectively. In the biological process analysis, all the 3 miRNAs were involved in transcription, DNA-templated and positive regulation of transcription from RNA polymerase II promoter. At the same time, the related pathways were involved in TGF-β signaling pathway, AMPK signaling pathway, Wnt signaling pathway, MAPK signaling pathway, cGMP-PKG and cAMP signaling pathway, etc.
Conclusion:
miR-192-5p, miR-194-5p and miR-1246 in serum exosomes might be a potential biomarker for AIDS combined with TM infection patients, which may be involved in TGF-β signaling pathway, AMPK signaling pathway, Wnt signaling pathway, MAPK signaling pathway, cGMP-PKG and cAMP signaling pathway, etc. Further research is needed on the biological functions and mechanisms of these miRNAs.
Insights
Three serum exosomal microRNAs (miRNAs) show potential as early diagnostic biomarkers for AIDS combined with Tuberculosis (TB) infection. These miRNAs, including miR-192-5p, miR-194-5p, and miR-1246, may aid in identifying coinfection.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Acquired Immunodeficiency Syndrome (AIDS) coinfection with Tuberculosis (TB) requires early diagnostic markers.
- Serum exosomal microRNAs (miRNAs) are investigated as potential biomarkers.
Purpose of the Study:
- To identify and analyze serum exosomal miRNAs for early diagnosis of AIDS combined with TB infection.
- To explore the diagnostic value of specific miRNAs in this coinfection.
Main Methods:
- RNA sequencing of serum exosomes from AIDS patients with and without TB infection.
- Validation using quantitative real-time polymerase chain reaction (qRT-PCR).
- Bioinformatic analysis to predict target genes and pathways.
Main Results:
- 131 differentially expressed serum exosomal miRNAs identified.
- miR-192-5p, miR-194-5p, and miR-1246 were upregulated in AIDS with TB patients.
- Combined ROC analysis showed an AUC of 0.742 for these three miRNAs.
Conclusions:
- miR-192-5p, miR-194-5p, and miR-1246 are potential biomarkers for AIDS combined with TB infection.
- These miRNAs are involved in key signaling pathways like TGF-β, AMPK, Wnt, MAPK, and cGMP-PKG/cAMP.
- Further research is needed to elucidate their biological functions and mechanisms.
