Related Experiment Video

Updated: Oct 11, 2025

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

6.3K

FOXC2 Alleviates Myocardial Ischemia-Reperfusion Injury in Rats through Regulating Nrf2/HO-1 Signaling Pathway

Rui Wang1, Yonggang Wu1, Shoutao Jiang1

  • 1Department of Cardiology, Guangzhou Hospital of Integrated Chinese and Western Medicine, Guangzhou, China.

Disease Markers
|December 3, 2021
PubMed
Abstract

Insights

Forkhead box C2 (FOXC2) protects against myocardial ischemia-reperfusion injury (MIRI) by reducing inflammation and oxidative stress. Upregulating FOXC2 promotes the Nrf2/HO-1 pathway, alleviating MIRI in rats.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Cell Biology

Background:

  • Myocardial ischemia-reperfusion injury (MIRI) is a primary cause of mortality in cardiovascular disease.
  • Understanding the molecular mechanisms underlying MIRI is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role and mechanism of forkhead box C2 (FOXC2) in mitigating MIRI in a rat model.
  • To elucidate how FOXC2 influences oxidative stress and inflammation in the context of MIRI.

Main Methods:

  • Establishment of rat MIRI models via ischemia-reperfusion surgery.
  • Assessment of myocardial structure and function using TTC staining, echocardiography, LDH assay, and H&E staining.
  • Detection of FOXC2 expression changes and its effect on oxidative stress, inflammation, and the Nrf2/HO-1 pathway in vivo and in vitro (H9c2 cells).

Main Results:

  • FOXC2 expression was significantly reduced in MIRI rats compared to controls.
  • FOXC2 overexpression attenuated myocardial damage, reduced serum inflammatory factors, and enhanced antioxidant capacity (SOD1/2) in MIRI rats.
  • FOXC2 promoted the Nrf2/HO-1 signaling pathway, which was essential for its protective effects on myocardial cells.

Conclusions:

  • Reduced FOXC2 expression is associated with MIRI in rats.
  • FOXC2 exerts a protective effect against MIRI by suppressing inflammation and oxidative stress.
  • The protective mechanism involves the activation of the Nrf2/HO-1 signaling pathway by FOXC2.

More Related Videos

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation09:53

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

Published on: January 10, 2025

595
Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control05:41

Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control

Published on: December 16, 2022

3.8K

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
5.3K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.9K