Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-speed full-field swept-source dynamic optical coherence tomography enabled by neural-network-based image generation.

Biomedical optics express·2026
Same author

Dynamic full-field swept-source optical coherence tomography for high-resolution, long-depth, and intratissue-activity imaging.

Biomedical optics express·2026
Same author

<i>In vivo</i> dynamic optical coherence tomography of human skin with hardware- and software-based motion correction.

Biomedical optics express·2026
Same author

Dynamic optical coherence microscope integrated with cell-cultivation chamber enabled longitudinal and early-stage assessment of tumor spheroid-drug interaction.

Scientific reports·2026
Same author

Dynamic optical coherence tomography algorithm for label-free assessment of swiftness and occupancy of intratissue moving scatterers.

Biomedical optics express·2026
Same author

Imaging of Tissue and Cell Dynamics: introduction to the feature issue.

Biomedical optics express·2026

Related Experiment Video

Updated: Oct 11, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.9K

Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation.

Ibrahim Abd El-Sadek1,2, Arata Miyazawa3, Larina Tzu-Wei Shen4

  • 1Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.

Biomedical Optics Express
|December 3, 2021
PubMed
Summary
This summary is machine-generated.

We developed a label-free 3D optical coherence tomography method to image tumor spheroid dynamics. This technique visualizes metabolic and necrotic activities, aiding in anti-cancer drug response assessment.

More Related Videos

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
10:13

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

Published on: June 21, 2022

2.3K
Three-Dimensional 3D Tumor Spheroid Invasion Assay
12:19

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

58.2K

Related Experiment Videos

Last Updated: Oct 11, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

7.9K
Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
10:13

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

Published on: June 21, 2022

2.3K
Three-Dimensional 3D Tumor Spheroid Invasion Assay
12:19

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

58.2K

Area of Science:

  • Biomedical Imaging
  • Optical Engineering
  • Cancer Research

Background:

  • Tumor spheroid models are crucial for studying cancer biology and drug efficacy.
  • Accurate visualization and quantification of tumor spheroid dynamics are essential for therapeutic development.
  • Current imaging methods often require labels or are time-consuming.

Purpose of the Study:

  • To present a novel, label-free, 3D optical coherence tomography (OCT) method for imaging tissue dynamics.
  • To enable visualization and quantification of metabolic and necrotic activities in tumor spheroids.
  • To assess anti-cancer drug responses using OCT-based dynamics imaging.

Main Methods:

  • Development of a custom 3D scanning protocol for rapid volumetric tissue dynamics tomography.
  • Application of the OCT method to tumor spheroid evaluation.
  • Qualitative visualization and quantitative analysis of time-course viability alterations.

Main Results:

  • The method successfully captured volumetric tissue dynamics in tens of seconds.
  • Time-course viability alterations and drug responses of tumor spheroids were visualized and quantified.
  • High similarity was observed between OCT-based dynamics images and fluorescence microscopy images.

Conclusions:

  • The label-free 3D OCT method provides a rapid and effective tool for analyzing tumor spheroid dynamics.
  • This technique offers a promising approach for evaluating metabolic/necrotic activities and anti-cancer drug responses.
  • The findings suggest OCT-based dynamics imaging as a valuable complement to traditional microscopy techniques.