Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

7.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same author

A mosaic of whole-body representations on the human precentral gyrus.

Nature·2026
Same author

A direct SCN-to-DMH output pathway organizes circadian behavioral timing.

bioRxiv : the preprint server for biology·2026
Same author

Encoding of speech modes and loudness in ventral precentral gyrus.

Nature communications·2026
Same author

Mechanistic studies on HNRNPA2B1 suggest binding but not selective recognition of m<sup>6</sup>A.

RNA biology·2026
Same author

Direct fluorescence detection and volume electron microscopy reveal a role for antibiotic biosynthesis in the bacterial cell envelope.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Oct 11, 2025

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.1K

Navigating the Light-Sheet Image Analysis Software Landscape: Concepts for Driving Cohesion From Data Acquisition to

Holly C Gibbs1,2, Sakina M Mota1, Nathan A Hart1

  • 1Department of Biomedical Engineering, Texas A&M University, College Station, TX, United States.

Frontiers in Cell and Developmental Biology
|December 3, 2021
PubMed
Summary

Selecting light-sheet microscopy tools can be challenging. This guide helps biologists choose appropriate imaging and analysis software by explaining computing concepts and reviewing current and future hardware and software options.

Keywords:
image analysislight-sheetmultiview deconvolutionparallel processingtool selection

More Related Videos

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
09:44

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Published on: June 13, 2018

8.3K
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.5K

Related Experiment Videos

Last Updated: Oct 11, 2025

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.1K
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
09:44

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Published on: June 13, 2018

8.3K
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.5K

Area of Science:

  • Microscopy and Imaging Science
  • Computational Biology
  • Bioinformatics

Background:

  • Light-sheet microscopy generates large datasets requiring specialized tools for effective analysis.
  • Interdisciplinary collaboration is crucial for addressing the complex challenges in light-sheet imaging and data processing.

Purpose of the Study:

  • To provide biologists with a framework for selecting appropriate light-sheet microscopy imaging and analysis tools.
  • To bridge the gap between biological research needs and computational infrastructure for data analysis.
  • To offer insights into current and future hardware and software solutions for light-sheet data.

Main Methods:

  • A multidisciplinary perspective from biologists, instrument developers, core facility scientists, and HPC experts.
  • Review of current hardware and software commonly used in light-sheet image analysis.
  • Discussion of basic computing concepts relevant to image data analysis.

Main Results:

  • Identification of key challenges in selecting light-sheet microscopy tools.
  • A framework for mapping light-sheet data peculiarities to computing environments and analysis pipelines.
  • An overview of existing tools and a perspective on future ideal solutions.

Conclusions:

  • Efficient selection of light-sheet microscopy tools requires understanding computational concepts and available resources.
  • The development of integrated hardware and software solutions is essential for advancing light-sheet data analysis.
  • Future tools should aim for greater accessibility and efficiency for biologists.