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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Updated: Oct 11, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Multiplexed Single-Cell in situ RNA Profiling.

Yu-Sheng Wang1, Jia Guo1

  • 1Biodesign Institute and School of Molecular Sciences, Arizona State University, Tempe, AZ, United States.

Frontiers in Molecular Biosciences
|December 3, 2021
PubMed
Summary
This summary is machine-generated.

Multiplexed fluorescence in situ hybridization (FISH) enables detailed RNA profiling in single cells, advancing health and disease research. This technique overcomes limitations of bulk analysis and conventional imaging for comprehensive spatial transcriptomics.

Keywords:
fishfluorescence in situ hybridizationgenomicstranscriptomicstranscripts

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biomedical Research

Background:

  • Bulk RNA analysis obscures cellular heterogeneity.
  • Conventional RNA imaging has limited multiplexing capabilities.
  • Spatial context is vital for understanding biological systems.

Purpose of the Study:

  • To review recent advances in multiplexed single-cell in situ RNA profiling.
  • To discuss the advantages, limitations, and applications of these technologies.
  • To identify current challenges and propose future solutions.

Main Methods:

  • Review of multiplexed fluorescence in situ hybridization (FISH) techniques.
  • Analysis of single-cell RNA profiling assays in their native spatial context.
  • Discussion of technological advancements in spatial transcriptomics.

Main Results:

  • Multiplexed FISH enables comprehensive RNA profiling at the single-cell level in situ.
  • These methods offer significant advantages over traditional approaches.
  • Wide applications exist in cell classification, network analysis, and disease diagnosis.

Conclusions:

  • Multiplexed single-cell in situ RNA profiling is a powerful tool for biological and biomedical research.
  • Continued technological development is crucial for overcoming current challenges.
  • These assays are poised to transform our understanding of health and disease.