Long non-coding RNA PVT1 facilitates cell migration and invasion by regulating miR-148a-3p and ROCK1 in breast cancer

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Breast cancer (BC) remains a leading cause of cancer-related mortality in women worldwide.
• Understanding the molecular mechanisms driving BC progression, particularly cell migration and invasion, is crucial for developing effective treatments.

Purpose of the Study:

• To investigate the role of long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) in breast cancer cell migration and invasion.
• To elucidate the underlying molecular mechanisms involving miR-148a-3p and Rho‑associated, coiled‑coil containing protein kinase 1 (ROCK1).

Main Methods:

• Quantitative real-time PCR (qRT-PCR) and Western blotting were used to assess PVT1, miR-148a-3p, and ROCK1 expression.
• Dual Luciferase activity, RNA immunoprecipitation (RIP), and Spearman correlation analyses were employed to determine molecular interactions.
• Transwell assays were conducted to evaluate cell migration and invasion capabilities.

Main Results:

• PVT1 and ROCK1 were found to be upregulated, while miR-148a-3p was downregulated in BC tissues and cell lines.
• miR-148a-3p directly binds to PVT1, and ROCK1 is identified as a direct target of miR-148a-3p.
• PVT1 was demonstrated to enhance BC cell migration and invasion by modulating miR-148a-3p and ROCK1 expression.

Conclusions:

• The study concludes that lncRNA PVT1 promotes breast cancer cell migration and invasion through the regulation of the miR-148a-3p/ROCK1 axis.
• These findings highlight PVT1 as a potential biomarker for BC diagnosis and a therapeutic target for BC treatment.