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Enzymatic Immunohistochemistry

Rodolfo Henrich Lobo1

  • 1Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA. RHenrichlobo@uams.edu.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2021
PubMed

Immunohistochemistry (IHC) is a powerful laboratory technique that is widely used in a variety of settings, including clinical medicine and research. Enzymatic immunohistochemistry refers to the process of detecting cell surface proteins or antigens using enzyme-labeled antibodies. The site and specificity of the antibody binding may be identified using direct labeling techniques or through secondary labeling methods. The antibody-antigen interaction is ultimately visualized using a chromogenic detection process, in which the enzyme conjugated to the antibody cleaves a substrate to produce a stable, colored precipitate.

Keywords:
IHCImmunohistochemical labelingImmunohistochemistryImmunoperoxidase

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