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Updated: Oct 11, 2025
11:13
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Published on: December 1, 2015
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Fluorescent Immunohistochemistry
Vanessa Moreno1, Elizabeth A Smith1, Sergio Piña-Oviedo2
1Arkana Laboratories, Little Rock, AR, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2021
Summary
Immunofluorescence is a sensitive technique using fluorescent antibodies to detect antigens in research and clinics. This guide details direct and indirect immunofluorescence methods and tissue preparation for accurate imaging.
Area of Science:
- Immunochemistry
- Cell Biology
- Histology
Background:
- Immunofluorescence is a key immunochemical technique for detecting specific antigens.
- It offers superior sensitivity and signal amplification compared to immunohistochemistry.
- Requires specialized fluorescence microscopy for sample analysis.
Purpose of the Study:
- To explain the principles of direct and indirect immunofluorescence.
- To provide guidance on preparing tissues for immunofluorescence labeling.
- To cover both fresh-frozen and formalin-fixed, paraffin-embedded tissues.
Main Methods:
- Detailed explanation of direct immunofluorescence principles.
- Detailed explanation of indirect immunofluorescence principles.
- Protocols for preparing fresh-frozen and FFPE tissues for immunofluorescence.
Main Results:
- Established protocols for tissue preparation.
- Demonstrated principles of direct and indirect immunofluorescence.
- Highlighted the importance of proper sample preparation for accurate results.
Conclusions:
- Immunofluorescence is a versatile and sensitive diagnostic and research tool.
- Proper tissue preparation is crucial for successful immunofluorescence.
- Understanding direct and indirect methods enhances experimental design.
Keywords:
Direct immunofluorescenceFFPEFresh-frozenImmunofluorescenceImmunohistochemistryIndirect immunofluorescenceMore Related Videos
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