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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

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Skinny deeping: Uncovering immune cell behavior and function through imaging techniques.

Yingrou Tan^1,2, Hong Liang Tey^2,3,4, Shu Zhen Chong¹

¹Singapore Immunology Network, Singapore, Singapore.

Immunological Reviews

|December 3, 2021

Summary

Skin immunity relies on immune cells like neutrophils, dendritic cells, and macrophages. New imaging techniques reveal their dynamic behavior, advancing systems biology understanding of skin immune surveillance and potential discoveries.

Keywords:

Ex vivo imaging In vivo imaging dendritic cells monocytes/macrophages neutrophils

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Area of Science:

Immunology
Dermatology
Systems Biology

Background:

The skin serves as a critical barrier, necessitating constant immune surveillance against external threats.
The innate immune system comprises the first responders to skin injury and infection.

Purpose of the Study:

To review key studies on immune cell behavior in the skin.
To discuss the importance of cellular interactions and functions in skin immunity from a systems biology perspective.
To explore emerging imaging technologies for advancing the understanding of skin immunity.

Main Methods:

Review of existing literature on neutrophil, dendritic cell, and macrophage behavior in skin.
Discussion of systems biology approaches to understanding immune cell interactions.
Overview of advanced imaging technologies: high-content proteomic screening, spatial transcriptomics, and 3D volumetric imaging.

Main Results:

Intravital imaging techniques enable detailed spatiotemporal analysis of immune cell responses in the skin.
Understanding immune cell dynamics highlights the importance of their interactions and functions.
Emerging technologies offer new avenues for a systems-level overview of skin immunity.

Conclusions:

Integrating advanced imaging and systems biology approaches is crucial for future discoveries in skin immunology.
Continued research into immune cell dynamics promises significant advancements in understanding and treating skin conditions.