Core-satellite assembly of gold nanoshells on solid gold nanoparticles for a color coding plasmonic nanosensor

We present core-satellite assemblies comprising a solid gold nanoparticle as the core and hollow decahedral gold nanoshells as satellites for tuning the optical properties of the plasmonic structure for sensing. The core-satellite assemblies were fabricated on a substrate via the layer-by-layer assembly of nanoparticles linked by DNA. We used finite-difference time-domain simulations to help guide the geometrical design, and characterized the optical properties and morphology of the solid-shell nanoparticle assemblies using darkfield microscopy, single-nanostructure spectroscopy, and scanning electron microscopy. Plasmon coupling yielded resonant peaks at longer wavelengths in the red to near-infrared range for solid-shell assemblies compared with solid-solid nanoparticle assemblies. We examined sensing with the solid-shell assemblies using adenosine triphosphate (ATP) as a model target and ATP-aptamer as the linker. Binding of ATP induced disassembly and led to a decrease in the scattering intensity and a color change from red to green. The new morphology of the core-satellite assembly enabled plasmonic color-coding of multiplexed sensors. We demonstrate this potential by fabricating two types of assemblies using DNA linkers that target different molecules - ATP and a model nucleic acid. Our work expands the capability of chip-based plasmonic nanoparticle assemblies for the analysis of multiple, different types of biomolecules in small sample sizes including the microenvironment and single cells.