Electrokinetic status of the subpopulations of lymph node cells in contact-sensitized CBA mice

Electrokinetic behaviour of LNC and their subpopulations from CBA mice contact-sensitized with dinitrofluorobenzene and oxazolone were studied. The sensitized LNC showed a significant reduction of mean EPM on the days of maximum DH response. Histogram analysis revealed that LNC of unsensitized as well as contact-sensitized mice were heterogeneous populations. While the LNC of unsensitized mice resolved into two subpopulations, sensitized LNC resolved into three distinct subpopulations. The emergence of a new populations with mean EPM intermediate between those of low and high mobility lymphocytes was a consequence of contact sensitization. Enriched subpopulations were also obtained by nylon adherence-dependent and surface marker-dependent procedures. histograms of these subpopulations revealed that in the mice sensitized to DNFB and oxazolone both T and B cells were electrokinetically altered and were heterogeneous in the distribution of their EPM. These findings are similar to the earlier observations on EPM of LNC in allograft-sensitized mice.