Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Area of Science:

• Biotechnology
• Microbial Fermentation
• Industrial Microbiology

Background:

• Rhizopus oryzae efficiently produces organic acids.
• Pellet formation in seed cultures enhances fermentation.
• Optimizing pellet morphology is crucial for industrial applications.

Purpose of the Study:

• To identify simple methods for inducing R. oryzae pellet formation.
• To understand the mechanisms controlling pellet morphology.
• To enhance organic acid fermentation efficiency.

Main Methods:

• Culturing R. oryzae in media supplemented with 1.5% triethanolamine (TEOA).
• Analyzing fermentation kinetics for organic acid production.
• Performing transcriptomic analysis to identify activated signaling pathways.

Main Results:

• 1.5% TEOA induced compact and uniform R. oryzae pellets in seed cultures.
• Fumaric and L-malic acid production increased by 293% and 177%, respectively.
• TEOA activated phosphatidylinositol and MAPK signaling pathways, stimulating cell wall synthesis.

Conclusions:

• TEOA is an effective agent for inducing optimal pellet morphology in R. oryzae.
• TEOA treatment enhances organic acid fermentation yields through specific molecular pathways.
• This study provides valuable insights for optimizing R. oryzae fermentation processes.