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Updated: Oct 11, 2025

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Concurrent mapping of multiple epigenetic marks and co-occupancy using ACT2-seq.

Benjamin Carter¹, Wai Lim Ku¹, Joe Pelt¹

¹Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, 20892, USA.

Cell & Bioscience

|December 5, 2021

Summary

This summary is machine-generated.

ACT2-seq enables simultaneous profiling of multiple epigenetic marks in single samples, directly mapping co-occupancy and revealing gene expression relationships. This method enhances epigenomic studies by improving efficiency and reducing costs.

Keywords:

ACT-seq Co-enrichment Co-occupancy Epigenetic marks Tagmentation

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Last Updated: Oct 11, 2025

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

ATAC-Seq Optimization for Cancer Epigenetics Research

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Published on: June 30, 2022

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

Area of Science:

Molecular genetics
Epigenomics
Genomics

Background:

Genome-wide profiling of epigenetic marks is crucial in molecular genetics.
Inferring co-occupancy of epigenetic marks often relies on multiple datasets, lacking direct evidence of co-enrichment within the same cells due to cellular heterogeneity.
Existing methods struggle to provide direct evidence of co-enrichment in single cells.

Purpose of the Study:

To develop a novel technique, ACT2-seq, for concurrently profiling multiple epigenetic marks within a single biological sample.
To enable direct mapping of epigenetic factor co-occupancy on chromatin.
To overcome limitations of existing methods in addressing cellular heterogeneity.

Main Methods:

Developed ACT2-seq, a technique for concurrent profiling of multiple epigenetic marks in one sample.
Applied ACT2-seq to profile pairs of two and three epigenetic marks simultaneously.
Validated the reproducibility and robustness of ACT2-seq data.

Main Results:

ACT2-seq successfully generated reproducible and robust data.
The technique effectively partitioned concurrently mapped datasets with distinct enrichment patterns.
Identified direct relationships between histone modification co-occupancy and gene expression patterns.

Conclusions:

ACT2-seq offers a user-friendly approach for epigenomic profiling.
The method has the potential to reduce sample numbers and sequencing costs.
ACT2-seq enables simultaneous profiling of co-occupancy for multiple histone marks and/or chromatin-associated proteins.