Carvacrol modulates the expression and activity of antioxidant enzymes in Candida auris

Outbreaks associated with Candida auris has notably increased around the globe. Being newly discovered, the evolutionary characteristics of this fungus are unexplored. The crucial feature associated with this pathogen is its multidrug resistance against the available antifungals, which renders a crucial need for developing novel therapeutic strategies. Activation of the antioxidant defence system has been reported as a common mechanism used by pathogens to escape drug toxicity. This system has also recently been recognized as an emerging antifungal drug target. Therefore, this study was conducted to assess the anti-Candida activity of carvacrol on the growth and survival of C. auris, gene expression and activity of antioxidant enzymes, as well as the effect on lipid peroxidation (LPO). The antifungal activity of carvacrol was determined using the microdilution method whereby the proliferation of C. auris was inhibited at an MIC range of 125-500 μg/mL. Spectrophotometric analysis revealed that carvacrol caused an adequate amount of oxidative stress which was clearly demonstrated by the significant increase in the activity and gene expression of primary antioxidant enzymes as well as LPO. This is the first study involving the antioxidant defence system of C. auris and provide attestation of oxidative stress induced by carvacrol in this pathogen.