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Updated: Oct 11, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Transforming heterotrophic to autotrophic denitrification process: Insights into microbial community, interspecific
Xiao Huang1, Chongsen Duan2, Jianghua Yu2
1Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China; Shenzhen Key Laboratory of Water Resources Utilization and Environmental Pollution Control, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
Abstract:
For investigating the microbial community, interspecific interaction and nitrogen metabolism during the transform process from heterotrophic to synergistic and autotrophic denitrification, a filter was built, and carbon source and sulfur concentration were changed to release the transformation process. The results demonstrated that the transformation process was feasible to keep nitrate nitrogen (NO3--N) discharge concentration lower than 15 mg L-1, however, nitrite nitrogen (NO2--N) accumulation and its rate reached 7.85% at initial stages. The dominant denitrification gunes were Methylophilaceae, Thiovulaceae and Hydrogenophilaceae for three processes, respectively, and the microbial interspecific interaction of heterotrophic denitrification was more complex than others. NO2--N accumulation was confirmed by the low abundance of EC1.7.7.1 and EC1.7.2.1, and the dominance degree of dark oxidation of sulfur compounds and dark sulfide oxidation improved in synthesis and autotrophic denitrifications.
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