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Updated: Oct 11, 2025

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Lorentz scanning electron/ion microscopy
Ken Harada1, Keiko Shimada1, Yoshio Takahashi2
1CEMS, RIKEN (Institute of Physical and Chemical Research), Hatoyama, Saitama 350-0395, Japan.
Abstract:
We have developed an observation and measurement method for spatial electromagnetic fields by using scanning electron/ion microscopes, combined with electron holography reconstruction technique. A cross-grating was installed below the specimen, and the specimens were observed under the infocus condition, and the grating was simultaneously observed under the defocus condition. Electromagnetic fields around the specimen were estimated from grating-image distortions. This method is effective for low and middle magnification and resolution ranges; furthermore, this method can in principle be realizable in any electron/ion beam instruments because it is based on the Lorentz force model for charged particle beams.
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