Related Experiment Video

Updated: Oct 11, 2025

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
08:03

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

20.2K

CDK5 inhibition improves glucose uptake in insulin-resistant neuronal cells via ERK1/2 pathway

Kapil Manglani1, Chinmoy S Dey1

  • 1Kusuma School of Biological Sciences, Indian Institute of Technology, Hauz Khas, New Delhi, India.

Cell Biology International
|December 5, 2021
PubMed

Insights

Inhibiting CDK5 enhances glucose uptake in insulin-resistant neuronal cells. This finding offers a new therapeutic approach for addressing neuronal insulin resistance and related conditions.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Metabolism

Background:

  • Cyclin-dependent kinase 5 (CDK5) plays a known role in neuronal functions.
  • The involvement of CDK5 in neuronal insulin signaling and insulin resistance remains unexplored.

Purpose of the Study:

  • To investigate the effect of CDK5 inhibition on neuronal insulin signaling, focusing on insulin-stimulated glucose uptake.
  • To explore the potential of CDK5 inhibition as a therapeutic strategy for neuronal insulin resistance.

Main Methods:

  • Developed an insulin-resistant neuro-2a cell model using chronic insulin treatment.
  • Assessed CDK5 expression levels in insulin-resistant cells.
  • Investigated the impact of CDK5 inhibition (using roscovitine) on basal and insulin-stimulated glucose uptake.
  • Examined the role of the ERK1/2 pathway in mediating the effects of CDK5 inhibition.

Main Results:

  • CDK5 expression increased in insulin-resistant neuro-2a cells.
  • CDK5 inhibition did not affect basal insulin signaling but enhanced insulin-stimulated glucose uptake in insulin-resistant cells.
  • CDK5 inhibition ameliorated insulin resistance and increased glucose uptake via the ERK1/2 pathway.

Conclusions:

  • CDK5 plays a significant role in neuronal insulin resistance.
  • Inhibiting CDK5 can improve glucose uptake in insulin-resistant neuronal cells.
  • CDK5 inhibition presents a novel therapeutic avenue for neuronal insulin resistance and associated disorders.
Keywords:
CDK5ERK1/2neuro-2aneuronal insulin resistanceneuronal insulin signalingroscovitine

More Related Videos

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

9.9K
High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds12:32

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

12.5K

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.9K
Insulin: The Receptor and Signaling Pathways01:28

Insulin: The Receptor and Signaling Pathways

Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
1.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K