Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K
Next-generation Sequencing03:00

Next-generation Sequencing

93.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
93.9K
DNA Microarrays02:34

DNA Microarrays

18.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.9K
Sanger Sequencing01:57

Sanger Sequencing

761.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
761.1K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
Synthetic Biology02:55

Synthetic Biology

5.0K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association Between Immunotherapy and Generalization in Ocular Myasthenia Gravis as a Function of Antibody Status.

Revista de neurologia·2026
Same author

Bclaf1 drives heart failure by recruiting Srsf2 to enhance Hand2 pre-mRNA splicing and pathological hypertrophy.

Nature communications·2026
Same author

Efficient semi-supervised estimation of optimal individualized treatment regimes with survival outcome.

Statistical methods in medical research·2026
Same author

Bridging the rural logistics penalty: quantifying structural costs and designing policy mechanisms for home TCM delivery to older adults with chronic respiratory diseases.

BMC health services research·2026
Same author

Resonant Acoustic Mixing-Assisted Fabrication of Fused Deposition Modeling-Printed Shape Memory P(DLLA-TMC)/β-TCP/PDA Composite Scaffolds Achieving Synergistic Osteogenic Effects.

ACS biomaterials science & engineering·2026
Same author

MXene/RGO/Si Schottky Junction for a High-Performance Self-Powered Broadband Photodetector.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Oct 11, 2025

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris
08:11

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Published on: January 3, 2025

620

Building a reference transcriptome for Juniperus squamata (Cupressaceae) based on single-molecule real-time

Yufei Wang1, Siyu Xie1, Jialiang Li1

  • 1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.

BMC Genomic Data
|December 6, 2021
PubMed
Summary

This study presents the first comprehensive transcriptome of Juniperus squamata, a key species in the Cupressaceae family. This resource aids future genomic research on conifers, overcoming challenges posed by their large genomes.

Keywords:
Gene ontology annotationJuniperus squamataSimple sequence repeatsSingle-molecule real-time sequencing

More Related Videos

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.2K
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

9.9K

Related Experiment Videos

Last Updated: Oct 11, 2025

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris
08:11

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Published on: January 3, 2025

620
Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.2K
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

9.9K

Area of Science:

  • Plant genomics
  • Conifer biology
  • Transcriptomics

Background:

  • Cupressaceae, a large conifer family, has significant ecological and economic value.
  • Large conifer genomes (>8 GB) hinder research, necessitating alternative genomic resources.
  • High-quality transcriptomes are crucial for gene discovery and annotation in non-model organisms.

Purpose of the Study:

  • To generate a comprehensive full-length transcriptome for Juniperus squamata, a major species within the Cupressaceae family.
  • To provide a foundational genomic resource for Juniperus species and the broader Cupressaceae family.
  • To facilitate future research into conifer genomics and evolutionary history.

Main Methods:

  • Single-molecule real-time (SMRT) sequencing was employed to obtain full-length transcriptome data.
  • Illumina RNA-seq data from the same individual was used for transcriptome correction.
  • Bioinformatic analyses included identification of simple sequence repeats (SSRs), open reading frames (ORFs), and functional annotation via Gene Ontology and InterPro databases.

Main Results:

  • A total of 47,860 non-redundant full-length transcripts were obtained, with an N50 of 2839.
  • 57,393 SSRs and 268,854 ORFs were identified.
  • 10,818 sequences were annotated in the Gene Ontology database, and 30,403 sequences were functionally characterized by InterPro analysis.

Conclusions:

  • This study provides the first comprehensive transcriptome characterization for Juniperus species.
  • The generated data serves as a vital reference for genomic and evolutionary studies within Cupressaceae and conifers.
  • This resource will accelerate gene discovery and annotation, aiding research into these ecologically important trees.