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JAZ8 Interacts With VirE3 Attenuating Agrobacterium Mediated Root Tumorigenesis
Shijuan Li1, Bingliang Xu1, Xiaolei Niu2
1College of Plant Protection, Gansu Agricultural University, Lanzhou, China.
Frontiers in Plant Science
|December 6, 2021
Summary
Agrobacterium tumefaciens VirE3 protein
Area of Science:
- Plant-microbe interactions
- Molecular plant pathology
- Plant defense signaling
Background:
- Agrobacterium tumefaciens causes crown gall tumors by transferring T-DNA and effector proteins.
- The VirE3 effector protein has multiple reported functions, including nuclear transcription regulation and plasma membrane interactions.
Purpose of the Study:
- To investigate the interaction between VirE3 and Arabidopsis JAZ8 (AtJAZ8).
- To elucidate the role of VirE3 and AtJAZ8 in Agrobacterium virulence and plant defense responses.
Main Methods:
- Yeast two-hybrid screening, pull-down assays, and bimolecular fluorescence complementation (BiFC) assays to confirm protein interactions.
- Agrobacterium root tumor assays to assess virulence.
- Gene expression analysis (RT-qPCR) of plant defense-related genes (AtPR1, AtPDF1.2) following overexpression of VirE3 and AtJAZ8.
Main Results:
- AtJAZ8 was identified as a VirE3-interacting protein.
- VirE3 enhances Agrobacterium tumorigenesis, while AtJAZ8 inhibits it.
- AtJAZ8 antagonizes VirE3's transcriptional regulatory activity and modulates plant defense signaling pathways (SA/JA).
Conclusions:
- A novel counter-defense mechanism employed by Agrobacterium is proposed, involving VirE3 and AtJAZ8.
- VirE3 and JAZ8 antagonistically regulate salicylic acid/jasmonic acid-mediated plant defense signaling during Agrobacterium infection.
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