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Quantitative Proteomics and Transcriptomics Reveals Differences in Proteins During Anthers Development in Oryza
Yue Sun1, Xin Wang1, Zhongkai Chen1
1Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Research Center of Super Rice Engineering and Technology, Jiangxi Agricultural University, Nanchang, China.
Frontiers in Plant Science
|December 6, 2021
Summary
African wild rice (Oryza longistaminata) has normal pollen vitality, despite self-incompatibility. Proteome analysis reveals active lipid and carbohydrate metabolism, crucial for improving hybrid rice breeding efficiency.
Area of Science:
- Plant Science
- Genetics
- Biochemistry
Background:
- Oryza longistaminata, an African wild rice, has valuable traits for breeding.
- Self-incompatibility is a primary cause of sterility in this species.
- Understanding pollen development and viability is key to its utilization.
Purpose of the Study:
- To investigate the underlying reasons for pollen viability in O. longistaminata.
- To analyze protein profiles related to metabolism during pollen development.
- To provide insights for enhancing hybrid rice breeding.
Main Methods:
- Pollen viability assessed using I2-KI and TTC staining.
- Aniline-blue staining for cytological analysis of pollen development.
- Tandem mass tags (TMT)-based quantitative proteomics and RNA-seq.
Main Results:
- Pollen exhibited normal germination in vitro and in vivo.
- Proteomic analysis revealed active lipid and carbohydrate metabolism.
- Fatty acid conversion to sucrose was observed post-6mm spikelet stage, involving glyoxylate cycle and gluconeogenesis enzymes.
Conclusions:
- Pollen vitality in O. longistaminata is supported by robust metabolic pathways.
- Proteome-level insights are crucial for understanding and improving its breeding potential.
- Findings can enhance male parent pollination efficiency in hybrid rice development.
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