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Related Experiment Video

Updated: Oct 11, 2025

piggyBac Transposon System Modification of Primary Human T Cells

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Published on: November 5, 2012

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.

Jonas Holst Wolff¹, Jakob Haldrup¹, Emil Aagaard Thomsen¹

¹Department of Biomedicine, Aarhus University, Aarhus, Denmark.

Frontiers in Genome Editing

|December 6, 2021

Summary

Prime editing offers precise genomic alterations but faces low efficacy. Our piggyPrime system enhances prime editing efficiency up to 100% using piggyBac DNA transposition for effective gene editing in human cells.

Keywords:

CRISPR DNA transposition pegRNA piggyBac prime editing prime editing guide RNA

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Last Updated: Oct 11, 2025

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Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Prime editing is an advanced genome editing tool enabling diverse genetic modifications.
Current prime editing methods often lack efficiency and rely on complex procedures.

Purpose of the Study:

To develop an efficient and easy-to-use system for prime editing in human cells.
To improve the efficacy of prime editing through genomic integration of its components.

Main Methods:

Development of piggyPrime, a single-vector system utilizing piggyBac DNA transposition.
Transfection of the piggyPrime system into human cells to integrate prime editing components.
Assessment of prime editing efficacy in various cell lines.

Main Results:

The piggyPrime system facilitates easy and effective transgenesis of prime editing components.
Achieved prime editing efficacies of up to 100% in tested cell lines.
Demonstrated the potential of piggyBac transposition for enhancing gene editing technologies.

Conclusions:

The piggyPrime system represents a significant advancement for efficient and reliable prime editing.
This novel approach overcomes the limitations of low efficacy in conventional prime editing.
piggyPrime offers a promising platform for diverse genomic applications in human cells.