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piggyBac Transposon System Modification of Primary Human T Cells
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piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
Jonas Holst Wolff1, Jakob Haldrup1, Emil Aagaard Thomsen1
1Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Frontiers in Genome Editing
|December 6, 2021
Abstract:
Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

