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Related Concept Videos

RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Related Experiment Video

Updated: Oct 11, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

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Single-Cell Sequencing for Everybody.

Valentina Proserpio1,2, Carlotta Duval3,4, Vanessa Falvo3,4

  • 1IIGM Foundation - Italian Institute for Genomic Medicine, Torino, Italy. valentina.proserpio@unito.it.

Methods in Molecular Biology (Clifton, N.J.)
|December 6, 2021
PubMed
Summary
This summary is machine-generated.

Smart-seq2 is a cost-effective protocol for full-length mRNA sequencing in single cells. It is adaptable for various cell types and laboratories, balancing cost with comprehensive transcript coverage.

Keywords:
Full lengthSingle cellSmart-seq2mRNA-seq

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Single-cell sequencing has gained significant traction across diverse biological fields, including neurobiology, developmental biology, immunology, and tumor biology.
  • The proliferation of protocols, instruments, and options presents a challenge for researchers new to single-cell analysis, particularly when investigating cellular heterogeneity.

Purpose of the Study:

  • To present and describe the Smart-seq2 protocol for full-length mRNA sequencing of single cells.
  • To provide a practical guide for laboratories seeking to implement single-cell sequencing techniques.

Main Methods:

  • Detailed description of the Smart-seq2 protocol.
  • Emphasis on its adaptability to standard molecular biology laboratory equipment.
  • Discussion of optimization strategies for various cell types.

Main Results:

  • The Smart-seq2 protocol is suitable for a wide range of cell types.
  • The protocol offers a relatively small cost per cell.
  • It achieves a favorable balance between experimental cost and the extent of transcript coverage.

Conclusions:

  • Smart-seq2 is an accessible and effective method for full-length single-cell mRNA sequencing.
  • The protocol empowers researchers to investigate cellular heterogeneity cost-effectively.
  • Its adaptability makes it a valuable tool for diverse biological research applications.