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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Related Experiment Video

Updated: Oct 11, 2025

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

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Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells.

Irene Zorzan1, Onelia Gagliano2,3, Nicola Elvassore2,3,4,5

  • 1Department of Biology, University of Padua, Padua, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|December 6, 2021
PubMed
Summary

We developed a novel, cost-effective method using messenger RNAs (mRNAs) and microfluidics to efficiently generate human induced pluripotent stem cells (iPSCs). This technique bypasses genetic modification for broader medical applications.

Keywords:
Induced pluripotent stem cellsMicrofluidicsPluripotencyReprogrammingmRNAs

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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Biotechnology

Background:

  • Human induced pluripotent stem cells (iPSCs) hold vast medical potential but traditional generation methods are slow, costly, and require genetic manipulation.
  • Naïve iPSCs represent a more primitive state with broader developmental potential, including placenta formation, compared to conventional primed iPSCs.

Purpose of the Study:

  • To develop an efficient, scalable, and cost-effective protocol for generating both naïve and primed human iPSCs.
  • To overcome limitations of viral vectors and stable genetic modifications in iPSC generation.

Main Methods:

  • Utilized messenger RNA (mRNA) delivery for reprogramming somatic cells.
  • Employed a microfluidic system for high-efficiency iPSC generation.
  • Described device fabrication and cell transfer protocols for subsequent culture expansion.

Main Results:

  • Achieved high-efficiency generation of both naïve and primed human iPSCs without stable genetic modification.
  • Demonstrated a reproducible and cost-effective protocol suitable for patient-specific iPSC production.
  • Successfully transferred iPSC colonies from microfluidic devices to standard multiwell plates.

Conclusions:

  • The developed mRNA-based microfluidic system offers a significant advancement for iPSC generation.
  • This method facilitates patient-specific iPSC production for cell therapy, disease modeling, and developmental studies.
  • The protocol is reproducible, cost-effective, and avoids genetic manipulation, enhancing iPSC accessibility.