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Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
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Lignin-based fluorescence-switchable graphene quantum dots for Fe3+ and ascorbic acid detection.

Lingyan Zhu1, Dongbing Li1, Heng Lu1

  • 1Key Comprehensive Laboratory of Forestry, College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China.

International Journal of Biological Macromolecules
|December 6, 2021
PubMed
Summary

This study presents an improved method for synthesizing lignin-based graphene quantum dots (GQDs) for detecting Fe3+ and ascorbic acid (AA). These GQDs offer high fluorescence stability and sensitivity for sensing applications.

Keywords:
Alkali ligninFluorescence sensingGraphene quantum dots

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Developing stable and high-performance graphene quantum dots (GQDs) from renewable resources like lignin is crucial for advanced sensing applications.
  • Existing synthesis methods often face challenges in achieving desired fluorescence stability, quantum yield, and selectivity.
  • Lignin-based GQDs offer a sustainable alternative to traditional carbon-based nanomaterials.

Purpose of the Study:

  • To develop an improved synthesis route for high-quality, nitrogen-doped graphene quantum dots (GQDs) from alkali lignin.
  • To evaluate the fluorescence stability, quantum yield, and biocompatibility of the synthesized GQDs.
  • To demonstrate the application of these GQDs for sensitive and selective detection of Fe3+ and ascorbic acid (AA).

Main Methods:

  • Utilized an acidolysis process with 17.5% nitric acid followed by hydrothermal treatment at 200°C for GQDs synthesis.
  • Characterized GQDs for fluorescence stability across various pH, duration, and NaCl concentrations.
  • Employed fluorescence spectroscopy for the detection of Fe3+ and AA, determining limits of detection and investigating sensing mechanisms.

Main Results:

  • Synthesized nitrogen-doped GQDs from alkali lignin with excellent fluorescence stability (pH 3-10, 1-12h, 0-1000mM NaCl) and a high quantum yield of 28%.
  • Achieved sensitive and selective fluorescence sensing of Fe3+ and AA with low limits of detection (1.49 μmol L⁻¹ for Fe3+, 1.62 μmol L⁻¹ for AA).
  • Elucidated sensing mechanisms involving GQDs-Fe3+ complex formation and Fe3+ reduction by AA.

Conclusions:

  • The developed acidolysis-hydrothermal method provides an efficient route to high-quality lignin-based GQDs.
  • These GQDs exhibit superior fluorescence properties and stability, making them suitable for biosensing.
  • The GQDs demonstrate significant potential for the selective and sensitive detection of Fe3+ and ascorbic acid in analytical applications.