Polysorbate-80 pretreatment contributing to volatile fatty acids production associated microbial interactions via...

Polyoxyethylene dehydration sorbitol monooleate (polysorbate-80) pretreatment enhanced volatile fatty acids (VFAs) production of waste activated sludge (WAS) in acidogenic fermentation. The results showed that polysorbate-80 ameliorated WAS solubilization obviously with a soluble chemical oxygen demand (SCOD) increasing to 1536 mg/L within 4 h. Within 2 days of acidogenic fermentation, the maximal VFAs arrived to 2958.35 mg COD/L via polysorbate-80-pretreatment. The polysorbate-80 pretreatment boosted microbial diversity and richness in fermentation process. The Clostridium, Macellibacteroides and Acidocella strengthened microbial cooperation for the metabolic functions enhancement (e.g. amino acid metabolism and carbohydrate metabolism) for VFAs generation from WAS organics. Overall, the polysorbate-80 could play positive roles on the transformation of organic matter from sludge solid matters to VFAs, which was turned out to become an effective enhancing strategy for future WAS treatment / bioresource recovery with relatively low cost.