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A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
07:01

A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model

Published on: September 11, 2020

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MicroRNA expression profiling in acute kidney injury

Akinori Aomatsu1, Shohei Kaneko2, Katsunori Yanai2

  • 1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama, Japan; Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama, Japan.

Translational Research : the Journal of Laboratory and Clinical Medicine
|December 6, 2021
PubMed

Insights

Researchers identified miRNA-5100 as a key regulator of acute kidney injury (AKI). Lowered miRNA-5100 levels in AKI patients suggest its potential as a diagnostic biomarker and therapeutic target.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nephrology

Background:

  • Acute kidney injury (AKI) is a critical clinical condition with limited diagnostic and therapeutic options.
  • MicroRNAs (miRNAs) are emerging as key regulators in various biological processes, including kidney injury.

Purpose of the Study:

  • To identify novel miRNAs involved in AKI pathogenesis.
  • To explore the diagnostic and therapeutic potential of identified miRNAs in AKI.

Main Methods:

  • Microarray analysis and quantitative RT-PCR were used to screen miRNA expression in two mouse models of AKI (LPS-induced and IRI-induced).
  • miRNA-5100 mimic conjugated with polyethylenimine nanoparticles (PEI-NPs) was administered to investigate therapeutic effects in vivo.
  • Serum miRNA levels were analyzed in AKI patients using ROC analysis.

Main Results:

  • miRNA-5100 expression was significantly decreased in the kidneys of both AKI mouse models.
  • Overexpression of miRNA-5100 using PEI-NPs inhibited apoptosis pathways and protected against renal ischemia-reperfusion injury (IRI-AKI) in mice.
  • Serum levels of miRNA-5100 were significantly lower in AKI patients compared to healthy subjects.
  • Serum miRNA-5100 demonstrated high accuracy in diagnosing AKI (AUC=0.96).

Conclusions:

  • miRNA-5100 plays a regulatory role in AKI.
  • miRNA-5100 is a promising diagnostic biomarker for AKI.
  • miRNA-5100 represents a potential therapeutic target for AKI treatment.
Keywords:
ADA = AdriamycinAKI = acute kidney injuryANOVA = one-way analysis of varianceBCIP = 5-bromo-4-chloro-3-indolyl phosphateBUN = blood urea nitrogenCRE = creatinineDAPI = 4′,6-diamidino-2-phenylindole-PBSGAPDH = glyceraldehyde 3-phosphate dehydrogenaseH&E = hematoxylin and eosinHIGA = high immunoglobulin AIGFBP-7 = insulin like growth factor binding protein 7IRI-AKI = ischemia-reperfusion injury-induced acute kidney injuryISH = In situ hybridizationKIM-1 = kidney injury molecule 1L-FABP = L-type fatty acid-binding proteinLPS-AKI = acute kidney injury induced by the intraperitoneal administration of lipopolysaccharideLPS = lipopolysaccharideNBT = nitroblue tetrazolium chlorideNGAL = neutrophil gelatinase-associated lipocalinPEI-NPs = polyethylenimine nanoparticlesRNU6 = U6 Small Nuclear 1TIMP-2 = tissue inhibitor of metalloproteinase 2UUO = unilateral ureteral obstructioneGFR = estimated glomerular filtration ratemRNA = messenger ribonucleic acidmiRNAs = micro ribonucleic acidqRT-PCR = quantitative real-time polymerase chain reaction

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