Related Experiment Video

Updated: Oct 11, 2025

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
10:03

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

Published on: January 28, 2020

5.5K

Opium may affect coronary artery disease by inducing inflammation but not through the expression of CD9, CD36, and

Mohammad Amin Momeni-Moghaddam1,2, Gholamreza Asadikaram3,4, Mohammad Masoumi5

  • 1Nutrition and Biochemistry, Gonabad University of Medical Sciences, Gonabad, Iran (the Islamic Republic of).

Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research
|December 7, 2021
PubMed

Insights

Opium addiction did not alter scavenger receptor expression (CD36, CD68, CD9) in coronary artery disease (CAD) patients. However, opium may contribute to CAD via inflammatory pathways, evidenced by increased tumor necrosis factor alpha (TNF-α) and malondialdehyde (MDA).

Area of Science:

  • Cardiovascular Disease Research
  • Molecular Biology
  • Immunology

Background:

  • Coronary Artery Disease (CAD) is a significant health concern with incompletely understood molecular underpinnings.
  • The specific impact of opium on CAD pathogenesis, particularly concerning inflammatory markers and scavenger receptor expression, remains largely undetermined.
  • Opium's complex chemical composition suggests potential interactions with cardiovascular disease pathways.

Purpose of the Study:

  • To investigate the effect of opium addiction on the expression of scavenger receptors (CD36, CD68, CD9) in monocytes of patients with CAD.
  • To assess plasma levels of key inflammatory markers, including tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), malondialdehyde (MDA), and nitric oxide metabolites (NOx), in CAD patients with and without opium addiction.
  • To elucidate the molecular mechanisms linking opium use to the development or progression of coronary artery disease.

Main Methods:

  • A case-control study design involving three groups: opium-addicted CAD patients (CAD+OA), non-addicted CAD patients (CAD), and a control group (Ctrl).
  • Quantification of scavenger receptor (CD9, CD36, CD68) protein and mRNA expression using flow cytometry and reverse transcription-quantitative PCR.
  • Measurement of plasma levels of TNF-α, IFN-γ, MDA, and NOx using appropriate biochemical assays.

Main Results:

  • Plasma levels of TNF-α were significantly elevated in the CAD+OA group compared to both CAD and Ctrl groups.
  • MDA levels were significantly increased in both CAD and CAD+OA groups relative to the Ctrl group.
  • No significant differences were observed in the expression of CD9, CD36, CD68, or plasma levels of IFN-γ and NOx across the three study groups.

Conclusions:

  • Opium addiction does not appear to significantly affect the gene or protein expression of CD36, CD68, and CD9 in monocytes of CAD patients.
  • Opium may contribute to CAD pathogenesis through inflammatory mechanisms distinct from the evaluated scavenger receptors, as indicated by elevated TNF-α.
  • Further research is warranted to explore alternative inflammatory pathways through which opium might influence the development of coronary artery disease.
Keywords:
coronary artery diseaseinflammation

More Related Videos

Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter07:53

Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter

Published on: June 16, 2022

3.4K
Flow Cytometry Analysis of Immune Cells Within Murine Aortas15:15

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Published on: July 1, 2011

34.9K

Related Concept Videos

Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
76
Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
473
Opioid Receptors: Overview01:22

Opioid Receptors: Overview

Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...
2.3K
Coronary Artery Disease III: Clinical Manifestations01:30

Coronary Artery Disease III: Clinical Manifestations

Coronary Artery Disease (CAD) is a primary health risk worldwide, leading to significant morbidity and mortality. The condition arises from the buildup of atherosclerotic plaques within the coronary arteries, resulting in diminished blood supply to the heart muscle.The clinical manifestations of CAD vary widely, from asymptomatic stages to severe, life-threatening conditions. Understanding these manifestations is crucial for early diagnosis and effective management.Angina Pectoris: The Warning...
64
Peripheral Artery Disease I: Introduction01:30

Peripheral Artery Disease I: Introduction

Peripheral artery disease (PAD) predominantly results from atherosclerosis, which involves the accumulation of fatty deposits, or plaques, within the walls of arteries. This causes them to narrow and harden, significantly reducing blood flow. PAD predominantly affects the legs, particularly the arteries supplying the thighs and calves. In rare cases, it may involve other arteries, including those in the arms.Etiology of PAD:The principal cause of PAD is atherosclerosis, which results from fatty...
65
Coronary Artery Disease IV: Preventive Measures01:26

Coronary Artery Disease IV: Preventive Measures

Effective preventive measures for coronary artery disease (CAD) focus on controlling modifiable risk factors, including cholesterol abnormalities and lifestyle changes.Cholesterol ManagementFirst, the Mediterranean diet and the American Heart Association advocate for maintaining low-density lipoprotein (LDL) cholesterol levels below 100 mg/dL, with a more stringent recommendation of below 70 mg/dL for individuals at high risk. LDL cholesterol, often termed "bad cholesterol," can lead to the...
388