Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

4.3K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
4.3K
Whole Body Regeneration01:33

Whole Body Regeneration

3.6K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
3.6K
Gastrulation01:56

Gastrulation

60.3K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
60.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell transcriptomics of X-ray irradiated <i>Drosophila</i> wing discs reveals heterogeneity related to cell-cycle status and cell location.

eLife·2026
Same author

The cell-adhesion molecule Echinoid promotes tissue survival and separately restricts tissue overgrowth.

Development (Cambridge, England)·2025
Same author

Single-cell transcriptomics of X-ray irradiated <i>Drosophila</i> wing discs reveals heterogeneity related to cell-cycle status and cell location.

bioRxiv : the preprint server for biology·2025
Same author

Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila.

Developmental biology·2024
Same author

Ets21C sustains a pro-regenerative transcriptional program in blastema cells of Drosophila imaginal discs.

Current biology : CB·2022
Same author

Single-cell transcriptomics of the <i>Drosophila</i> wing disc reveals instructive epithelium-to-myoblast interactions.

eLife·2021

Related Experiment Video

Updated: Oct 11, 2025

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
11:12

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Published on: November 1, 2013

12.6K

Imaginal Disc Regeneration: Something Old, Something New.

Melanie I Worley1, Iswar K Hariharan1

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA.

Cold Spring Harbor Perspectives in Biology
|December 7, 2021
PubMed
Summary

Drosophila imaginal disc regeneration involves wound healing and cell fate changes. Recent genetic tools reveal how early wound responses and gene regulation limit regeneration as tissues mature.

More Related Videos

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

27.0K
Long-term Live Imaging of Drosophila Eye Disc
06:08

Long-term Live Imaging of Drosophila Eye Disc

Published on: May 6, 2017

8.6K

Related Experiment Videos

Last Updated: Oct 11, 2025

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
11:12

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Published on: November 1, 2013

12.6K
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

27.0K
Long-term Live Imaging of Drosophila Eye Disc
06:08

Long-term Live Imaging of Drosophila Eye Disc

Published on: May 6, 2017

8.6K

Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Genetics

Background:

  • Imaginal discs in Drosophila larvae generate adult structures like wings and legs.
  • Early regeneration studies relied on challenging transplantation experiments, yet characterized key processes like wound healing and transdetermination.
  • Recent advances utilize genetic tissue-ablation systems for more reproducible and genetically informative research.

Purpose of the Study:

  • To explore the molecular mechanisms underlying Drosophila imaginal disc regeneration.
  • To understand how early wound responses influence proliferation and regenerative capacity.
  • To identify genetic factors controlling cellular plasticity during regeneration.

Main Methods:

  • Utilizing advanced genetic tissue-ablation systems in Drosophila.
  • Analyzing genetic and molecular pathways involved in wound healing and cell fate.
  • Investigating enhancer activity and gene expression changes during regeneration.

Main Results:

  • Mechanistic links established between early wound responses and proliferation signaling pathways.
  • Discovered localized silencing of damage-responsive enhancers limits regenerative capacity in mature tissues.
  • Identified genes crucial for maintaining cellular plasticity within regulated boundaries during regeneration.

Conclusions:

  • Modern genetic tools have significantly advanced the study of imaginal disc regeneration.
  • Regenerative capacity is tightly regulated by wound-induced signaling and developmental stage.
  • Understanding these mechanisms offers insights into controlling tissue repair and development.