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Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
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A genetically encoded fluorescent biosensor for extracellular L-lactate.

Yusuke Nasu1, Ciaran Murphy-Royal2,3, Yurong Wen4,5

  • 1Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.

Nature Communications
|December 7, 2021
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Summary
This summary is machine-generated.

Researchers developed a new biosensor, eLACCO1.1, to visualize extracellular L-lactate. This tool helps study L-lactate's role as an energy shuttle between cells in mammals.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Neuroscience

Background:

  • L-Lactate is increasingly recognized as a key intercellular energy shuttle in mammals.
  • Understanding extracellular L-lactate dynamics is crucial for investigating its emerging biological roles.

Purpose of the Study:

  • To develop a novel biosensor for real-time imaging of extracellular L-lactate.
  • To facilitate research into the intercellular transport and function of L-lactate.

Main Methods:

  • An intensiometric green fluorescent genetically encoded biosensor, eLACCO1.1, was engineered.
  • The biosensor was validated in cultured mammalian cells and ex vivo brain tissue.

Main Results:

  • eLACCO1.1 successfully enabled cellular resolution imaging of extracellular L-lactate.
  • The biosensor provides a new tool for visualizing L-lactate dynamics in biological systems.

Conclusions:

  • The eLACCO1.1 biosensor is a valuable tool for studying extracellular L-lactate.
  • This advancement will aid in understanding L-lactate's role in cellular energy metabolism and intercellular communication.