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Related Experiment Video

Updated: Oct 11, 2025

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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Functional interplay within the epitranscriptome: Reality or fiction?

Lina Worpenberg1, Chiara Paolantoni1, Jean-Yves Roignant1,2

  • 1Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|December 7, 2021
PubMed
Summary
This summary is machine-generated.

RNA modifications regulate gene expression, with N6-methyladenosine being key. This review explores the interplay between various RNA modifications and their combined impact on gene regulation, highlighting emerging research areas.

Keywords:
2′-O-methylationRNA modificationsepitranscriptomicsinosinem5Cm6Apseudouridine

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • RNA modifications represent a crucial regulatory layer influencing gene expression.
  • N6-methyladenosine (m6A) is the most prevalent mRNA modification, impacting RNA metabolism and disease.
  • Other modifications like 5-methylcytosine, N1-methyladenosine, RNA editing, 2'-O-methylation, and pseudouridine also play significant roles.

Purpose of the Study:

  • To review the current understanding of individual RNA modifications.
  • To explore the interplay between different RNA modifications in gene expression control.
  • To highlight emerging research and future directions in the field of RNA modification crosstalk.

Main Methods:

  • Literature review of existing studies on RNA modifications.
  • Analysis of examples demonstrating the interplay between different RNA modifications.
  • Discussion of technological advancements in mapping RNA modifications.

Main Results:

  • Significant progress has been made in understanding the individual roles of various RNA modifications.
  • Examples of interplay between RNA modifications are emerging, suggesting complex regulatory networks.
  • The dysregulation of RNA modifications is linked to numerous diseases.

Conclusions:

  • While individual RNA modification functions are increasingly understood, their combined effects are less clear.
  • The interplay of RNA modifications is a critical, yet understudied, aspect of gene expression regulation.
  • Advancements in mapping technologies are expected to accelerate the discovery of more interplay examples.