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Pregnane glycosides from Adonis amurensis and their bioactivity
Yue Yan1, Di Zhou1, Xuezheng Li2
1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Abstract:
Seven undescribed pregnane glycosides named amurensides A-G and two known aglycones were isolated from the whole herb of Adonis amurensis Regel & Radde. Their structures were established based on 1D and 2D NMR spectroscopic analyses, high-resolution mass spectrometry, and acid hydrolysis. The cytotoxicity of all compounds against three tumor cell lines (HepG2, Caco-2, and A549) were evaluated. Among them, amurensides A-C and E showed moderate inhibitory effects on the growth of HepG2 cells, with IC50 values ranging from 15.6 to 48.7 μM (sorafenib, 7.5 ± 1.9 μM). Amurensides A、D and F displayed inhibitory effects on the growth of A549 cells with IC50 values of 18.8 ± 1.2, 12.4 ± 0.6, and 30.4 ± 0.1 μM (cis-platinum, 6.1 ± 0.1 μM), respectively.
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