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Related Experiment Videos

Changes in phospholipid metabolism during B lymphocyte activation.

M K Kriz, E S Vitetta, T J Sullivan

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 15, 1986
    PubMed
    Summary

    Stimulating murine B lymphocytes with anti-immunoglobulin (anti-Ig) triggers rapid, specific changes in phospholipid metabolism, particularly phosphatidic acid and phosphatidylinositol. These metabolic shifts correlate with increased B cell proliferation, highlighting a key signaling pathway.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • B lymphocytes play a crucial role in adaptive immunity.
    • Immunoglobulin (Ig) signaling is central to B cell activation and function.
    • Phospholipid metabolism is critical for cellular signaling and proliferation.

    Purpose of the Study:

    • To investigate the effects of anti-Ig stimulation on phospholipid metabolism in murine B lymphocytes.
    • To determine if changes in phospholipid metabolism correlate with B cell proliferation.
    • To examine these responses in both normal and neoplastic B cells.

    Main Methods:

    • Culturing T cell-depleted splenic B lymphocytes with Sepharose-coupled goat anti-mouse Ig (GAMIg).
    • Measuring the incorporation of 32PO4 into various phospholipids and [3H]-thymidine uptake.

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  • Utilizing negative and positive selection to confirm responses in B lymphocytes.
  • Investigating neoplastic B lymphocytes (BCL1) with different Sepharose-bound antibodies.

    • Main Results:

    • GAMIg stimulation rapidly increased 32PO4 incorporation into phosphatidic acid and phosphatidylinositol in B lymphocytes.
    • A significant increase in [3H]-thymidine uptake was observed at 48 hours post-stimulation.
    • These phospholipid changes occurred selectively and did not require accessory cells or cytokines.
    • Similar selective phospholipid metabolism changes and dose-response relationships were observed in BCL1 cells stimulated with Sepharose anti-mu.

    Conclusions:

    • Interaction of B lymphocytes with insolubilized anti-Ig induces prompt and selective alterations in phospholipid metabolism.
    • These metabolic changes are strongly correlated with B lymphocyte proliferation.
    • The findings provide insights into the early signaling events governing B cell activation and growth.