Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of NIST Standard Reference Material 2082, a Pathlength Standard for Measurements in the Ultraviolet

Brian Lang1, Aaron Urbas1, Paul DeRose1

  • 1National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Journal of Research of the National Institute of Standards and Technology
|December 8, 2021
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An embryo editing 'first' is more complicated than headlines suggest.

Science (New York, N.Y.)·2026
Same author

Development, characterization, and inter-laboratory validation of methylated human cell free DNA candidate reference materials.

Clinical epigenetics·2026
Same author

Cellular 'vaults' deployed to spy on gene activity.

Science (New York, N.Y.)·2026
Same author

Functional Characterization and a Real-World Clinical Laboratory Pilot of the Foundation for the National Institutes of Health Circulating Tumor DNA Quality Control Materials.

JCO precision oncology·2025
Same author

Supreme Court quashes restoration of NIH grants.

Science (New York, N.Y.)·2025
Same author

Correction: Development and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pair.

Scientific data·2025

New spectrophotometers and cuvettes enable absorbance measurements of small sample volumes. A new standard, SRM 2082, validates these short pathlength instruments for protein and nucleic acid analysis.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biochemistry

Background:

  • Microliter volume measurements require specialized spectrophotometers and cuvettes with short pathlengths.
  • Accurate absorbance measurements are crucial for analyzing precious samples like proteins and nucleic acids.
  • Existing ultraviolet absorbance standards are designed for longer pathlengths, limiting their use with new microvolume instruments.

Purpose of the Study:

  • To develop and validate a new Standard Reference Material (SRM) for short pathlength spectrophotometry.
  • To provide a reliable standard for calibrating instruments used with microliter sample volumes.
  • To ensure accurate absorbance measurements for biomolecules like proteins and nucleic acids.

Main Methods:

  • Development of SRM 2082, comprising a blank buffer, tryptophan solution (protein mimic), and uracil solution (nucleic acid mimic).
Keywords:
absorbancecertified reference materialnucleic acidpathlengthproteinspectrophotometerstandardvisible UV

Related Experiment Videos

  • Measurement of absorbance values using cuvettes with pathlengths ranging from 0.1 mm to 2 mm.
  • Determination of cuvette pathlengths via physical and optical methods at the National Institute of Standards and Technology.
  • Investigation of temperature and spectral bandwidth effects on absorbance values.

    • Main Results:

    • SRM 2082 provides absorbance spectra similar to proteins (peak at 280 nm) and nucleic acids (peak at 260 nm).
    • The standard was used to validate short pathlength cuvettes and spectrophotometers.
    • Absorbance values were accurately determined across a range of short pathlengths.
    • Temperature and spectral bandwidth effects were quantified.

    Conclusions:

    • SRM 2082 is effective for validating short pathlength cuvettes and spectrophotometers.
    • This new standard supports accurate microvolume absorbance measurements for biomolecules.
    • The development enhances the reliability of spectrophotometric analysis for limited-volume samples.