Related Experiment Video
Updated: Oct 10, 2025

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
Published on: June 23, 2023
Applications of infrared spectroscopy in polysaccharide structural analysis: Progress, challenge and perspective
Tao Hong1, Jun-Yi Yin1, Shao-Ping Nie1
1State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.
Infrared (IR) spectroscopy is a key technique for analyzing polysaccharide structures. Advanced IR methods, combined with mass spectrometry and microscopy, enhance carbohydrate characterization and industrial applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Spectroscopy
Background:
- Polysaccharides are crucial biomacromolecules with diverse functions and industrial uses.
- Their properties are intrinsically linked to their unique structural features.
- Infrared (IR) spectroscopy is a standard method for analyzing polysaccharide structures.
Purpose of the Study:
- To review the principles and applications of IR spectroscopy in polysaccharide structural analysis.
- To highlight classical and advanced IR techniques for carbohydrate characterization.
- To emphasize the impact of integrating IR with other methods like mass spectrometry and microscopy.
Main Methods:
- Review of established IR spectroscopy principles and spectrum interpretation.
- Analysis of qualitative and quantitative applications of IR in polysaccharide elucidation.
- Introduction to advanced IR techniques integrated with mass spectrometry (MS), microscopy, and computational chemistry.
Main Results:
- IR spectroscopy provides valuable insights into polysaccharide structures.
- Classical IR methods offer both qualitative and quantitative analysis.
- Advanced IR techniques significantly expand the scope of carbohydrate characterization.
Conclusions:
- IR spectroscopy is indispensable for understanding polysaccharide structures.
- Integrating IR with complementary techniques like MS and microscopy offers powerful analytical capabilities.
- These advanced approaches are vital for advancing carbohydrate science and applications.
More Related Videos
Related Concept Videos
Applications of IR Spectroscopy: Overview
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
Biosynthesis of Polysaccharides
Spectroscopy of Carboxylic Acid Derivatives
IR and UV–Vis Spectroscopy of Carboxylic Acids
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
Chemistry of Carbohydrates

