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Updated: Oct 10, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)
Nupur Sharma1, Sadam H Bhat1, Gaurav Tripathi1
1Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
Abstract:
Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
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