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Atomic Fluorescence Spectroscopy01:29

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Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
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Ascorbic acid detector based on fluorescent molybdenum disulfide quantum dots.

Yaping Zhong1, Yibiao Zou2, Xianhong Yang2

  • 1Key Laboratory of Textile Fiber and Products, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials & Application, Wuhan Textile University, Wuhan, 430200, China. ypzhong@wtu.edu.cn.

Mikrochimica Acta
|December 8, 2021
PubMed
Summary

This study introduces a fast and easy way to detect ascorbic acid (AA) using molybdenum disulfide quantum dots (MoS2 QDs). The MoS2 QDs act as a fluorescence sensor, enabling sensitive and selective AA detection in various samples.

Keywords:
Ascorbic acidFluorescence sensorLiving cells imagingMolybdenum disulfide quantum dots

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Ascorbic acid (AA) is a vital nutrient with diverse biological roles.
  • Accurate and rapid detection methods for AA are crucial in food, clinical, and biological research.
  • Existing detection methods may lack sensitivity, selectivity, or speed.

Purpose of the Study:

  • To develop a rapid, facile, and highly selective fluorescence sensing method for ascorbic acid (AA).
  • To utilize molybdenum disulfide quantum dots (MoS2 QDs) as a novel fluorescence sensor for AA detection.
  • To explore the application of this sensing strategy in real-world samples and biological imaging.

Main Methods:

  • Synthesis of water-soluble and biocompatible MoS2 QDs via a hydrothermal method.
  • Construction of a fluorescence sensing mechanism based on the modulation of MoS2 QD fluorescence by Fe3+ ions and AA.
  • Utilizing the redox reaction between AA and Fe3+ to quantify AA concentration through fluorescence quenching/restoration.

Main Results:

  • Successfully synthesized MoS2 QDs with maximum fluorescence emission at 506 nm.
  • Established a fluorescence quenching mechanism involving Fe3+ and demonstrated AA's ability to restore fluorescence.
  • Achieved a good linear relationship for AA detection in the range of 1–150 μM with a low detection limit of 50 nM.
  • Demonstrated high selectivity, simplicity, and rapidity (5 min at room temperature).
  • Validated the method in fruit, beverage, and serum samples, and performed AA imaging in living cells.

Conclusions:

  • The developed MoS2 QD-based fluorescence sensing strategy offers a highly selective, simple, and rapid method for AA detection.
  • The method shows significant potential for practical applications in food analysis, clinical diagnostics, and biological imaging.
  • This work highlights the versatility of MoS2 QDs in developing advanced fluorescence detection platforms.