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Bisulfite-free and quantitative detection of 5-formylcytosine in DNA through qPCR
Jizhou Liu1, Wei Yang1, Xiong Zhang1
1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
Chemical Communications (Cambridge, England)
|December 8, 2021
Abstract:
An easily operated bisulfite-free method was presented to detect and quantify 5fC through quantitative real-time PCR. Malononitrile can selectively label 5fC under mild reaction conditions causing a C-to-T conversion that affects the nick ligation of the complementary pairing oligos, and then the ligation product is amplified and visualized by qPCR.
